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Recent genetic studies have revealed out the utility of recombinant adeno-associated viral, abbreviated as rAAV, which are vectors in the generation of human knockout cell lines. The efficacy of generation of such cell lines can occur with the use of rAAV, when compared with elaborately described plasmid-based approaches, has not been tested directly.
This report demonstrated that rAAV vectors delivered targeting constructs were nearly 25times more powerful than transfected plasmids which target the same exon. Apart from this, configuration of a novel vector was describe which was known as the synthetic exon promoter trap, abbreviated as SEPT. This targeting element further brought an improvement in the efficiency of knockout generation and also made easy the generation of knock in changes.
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