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Examples of endotoxins are the molecules of lipopolysaccharide present in the wall of the cell of gram-negative bacteria; and are usually released following the lysis of the bacteria. These endotoxins become pyrogenic, that is they cause fever when they are released into the blood stream. Though numerous gram-negative are found normally in the intestine of human beings, they do not cause any harm, because they do not undergo lysis and moreover the endotoxins are not produced as long as the intestinal lining is intact.
Since the molecular weight of endotoxin may differ a great deal ranging from 10,000 to 1,000,000 Da, the level of endotoxins are measured in endotoxin units, which is commonly abbreviated as EU. One EU is approximately equal to 100 pg of the gram-negative bacteria, E. Coli lipopolysaccharide which means approximately equal to the amount present in around 105 bacteria. Humans usually develop symptoms on exposure to as little as 5 EU/kg of body weight. Some of these symptoms include, fever, hypotension, tachycardia, and oliguria; and sometimes even small amounts of endotoxin in the blood may have a fatal result.
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